Male Fraction (Sperm Fraction)
Definition
The lysate produced by the second (DTT + proteinase K) lysis step after washing the sperm pellet. Ideally contains predominantly the suspect's sperm-cell nuclear DNA.
Related terms
- Differential Extraction
- A two-step DNA extraction procedure used when a stain contains both sperm cells and non-sperm epithelial cells. The first step lyses epithelial...
- DTT (Dithiothreitol)
- A reducing agent that cleaves the disulphide bonds in the sperm protamine matrix, allowing proteinase K and SDS to lyse the sperm...
- Erase Sperm Isolation Kit
- An immunomagnetic sperm-cell isolation system using paramagnetic beads coated with antibodies against sperm-specific surface antigens. Achieves greater than 99% sperm recovery with...
- Female Fraction
- The lysate produced by the first (no-DTT) lysis step of differential extraction. Contains DNA from epithelial cells and other non-sperm cellular material...
- Laser Capture Microdissection (LCM)
- A UV-laser instrument technique for physically isolating individual cells or clusters of cells from a stained slide into a capture tube, without...
- Protamine
- Small arginine-rich proteins that replace histones in the nuclei of spermatozoa during spermatogenesis, producing a hyper-compacted chromatin structure approximately six times denser...
Explained in
- Differential Extraction in Sexual-Assault CaseworkThe lysate produced by the second (DTT + proteinase K) lysis step after washing the sperm pellet. Ideally contains predominantly the suspect's sperm-cell nucle...