Lattes Crust Method
Definition
Classical direct agglutination. A crumb of the dried crust is teased into a drop of known antiserum on a slide. Leone Lattes, Turin, 1915.
Related terms
- Absorption-Elution
- A technique for detecting antigens on dried red cell debris. Specific antiserum is absorbed onto the stain; if the antigen is present,...
- Absorption-Inhibition
- A known volume of antiserum is exposed to the stain; the residual unbound antibody is then titrated against indicator cells. A drop...
- Agglutination
- Clumping of red cells that occurs when antibodies in serum bind to incompatible antigens on the cell surface, cross-linking cells into visible...
- Antiserum
- Serum collected from an immunised animal containing antibodies directed against a specific antigen or group of antigens. In species identification, antiserum raised...
- Mixed Agglutination
- A microscopy-based technique in which indicator cells form rosette aggregates around antigen-bearing stain material via antibody bridges. Positive results are read as...
- Secretor Status
- A genetic trait that determines whether a person expresses their ABO blood group antigens in secreted body fluids such as saliva, semen,...
- Sensitivity Threshold
- The smallest stain on which a technique still gives a reliable read. The key axis for choosing between Lattes, inhibition, elution and...
Explained in
- Techniques for Determining Blood Groups from BloodstainsClassical direct agglutination. A crumb of the dried crust is teased into a drop of known antiserum on a slide. Leone Lattes, Turin, 1915.