Lateral-flow immunoassay (LFIA)
Definition
A rapid immunological test format using a nitrocellulose membrane strip. An extracted sample migrates by capillary action past antibody-conjugate and capture zones, producing a coloured band when the target antigen is present. Results are available in 5 to 15 minutes without laboratory equipment.
Related terms
- Cross-reactivity
- The capacity of an antibody raised against one analyte to bind structurally related compounds. In RIA, cross-reactivity is the main driver of...
- Dermcidin
- An antimicrobial peptide secreted constitutively by eccrine sweat glands. The primary candidate marker for immunological identification of sweat in forensic samples, though...
- Human leucocyte elastase (HLE)
- A serine protease secreted by vaginal epithelial cells and leucocytes. The primary immunological target for forensic identification of vaginal secretions, detected using...
- Matrix metalloproteinase-10 (MMP-10)
- A zinc-dependent endopeptidase present in menstrual blood but not in peripheral blood. The principal marker used in lateral-flow assays designed to distinguish...
- Tamm-Horsfall protein (uromodulin)
- The most abundant protein in normal human urine, secreted exclusively by cells of the thick ascending limb of Henle's loop in the...
Explained in
- Immunological Identification of Vaginal Secretions, Urine, and Other FluidsA rapid immunological test format using a nitrocellulose membrane strip. An extracted sample migrates by capillary action past antibody-conjugate and capture z...