Inner-Filter Effect
Definition
Attenuation of excitation or emitted light within a specimen that can distort measured luminescence.
Related terms
- Fluorescence
- Light emission following absorption of light at a shorter (higher-energy) wavelength. Fluorescein absorbs blue-green excitation light and emits yellow-green light. The emitted...
- Jablonski Diagram
- An energy-level diagram showing the ground state, singlet excited states, and triplet state of a molecule, with arrows indicating absorption, internal conversion,...
- Phosphorescence
- Light emission from the triplet excited state (T1) via a spin-forbidden radiative transition; characterised by emission persisting after the excitation source is...
- Quantum Yield
- The number of events of a specified type per photon absorbed; fluorescence quantum yield counts fluorescence photons.
- Stokes Shift
- The wavelength difference between the excitation peak and the emission peak of a fluorophore. A positive Stokes shift (emission at longer wavelength...