Enzyme conjugate
Definition
An antibody (or antigen) chemically linked to a reporter enzyme, most commonly horseradish peroxidase (HRP) or alkaline phosphatase (AP). The enzyme catalyses a colour-generating reaction in the detection step, linking the immunological binding event to a measurable optical signal.
Related terms
- Competitive ELISA
- An ELISA format in which sample antigen competes with a fixed quantity of labelled antigen for a limited number of antibody binding...
- Cross-reactivity
- The capacity of an antibody raised against one analyte to bind structurally related compounds. In RIA, cross-reactivity is the main driver of...
- Sandwich ELISA
- An ELISA format in which a capture antibody immobilised on the plate binds one epitope of the target antigen, and a separate...
- Solid phase
- The inert surface to which an antigen or antibody is adsorbed at the start of an ELISA. Usually the well walls of...
- Substrate
- The compound converted by the reporter enzyme into a coloured product. For HRP, 3,3',5,5'-tetramethylbenzidine (TMB) is the standard substrate, producing a blue...
Explained in
- ELISA: Principles, Formats, and Forensic ApplicationsAn antibody (or antigen) chemically linked to a reporter enzyme, most commonly horseradish peroxidase (HRP) or alkaline phosphatase (AP). The enzyme catalyses...