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Ehrlich's Reagent

Definition

A solution of para-dimethylaminobenzaldehyde in acid. It reacts with urobilinogen and related pyrrole-containing compounds to produce a pink-red colour, used as a chemical test for faecal material in forensic screening.

Active reagent
Para-dimethylaminobenzaldehyde in acid
Target compound
Urobilinogen and pyrrole compounds
Positive colour
Pink-red
Application
Presumptive faecal stain screening

Common questions

Why is urobilinogen a useful marker for faecal material specifically?+

Urobilinogen is a breakdown product of bile pigment produced by gut bacteria and is characteristically concentrated in faeces, so its presence supports faecal origin for a stain in a way that general protein or blood tests cannot.

Can Ehrlich's reagent be confused with the test of the same name used for indole alkaloids?+

Yes, the name is shared with the presumptive drug screen for LSD and related indoles, which uses the same core chromogen but is applied to a different sample type and target analyte, so case notes should specify which application was performed.

Related terms

Ferning
The arborisation pattern formed when cervical or vaginal mucus dries on a glass slide, produced by sodium chloride and glycoprotein crystallisation. Visible...
Glycogen
A polysaccharide energy store present in vaginal epithelial cells. It is released when cells are lysed and can be detected by periodic...
Periodic Acid-Schiff (PAS) Reaction
A histochemical staining method that oxidises polysaccharides (including glycogen) to produce aldehydes, which are then detected by Schiff reagent as a magenta...
RSID-Vaginal Mucosa
Rapid Stain Identification of Vaginal Mucosa. An immunochromatographic lateral-flow assay using antibodies targeting proteins specific to human vaginal epithelial cells, providing higher...
Urobilinogen
A colourless bile pigment metabolite produced by intestinal bacteria from bilirubin. It is excreted in faeces (as urobilin/stercobilin) and detected in forensic...

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