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Big Dye Terminator (BDT) v3.1

Applied Biosystems' (now Thermo Fisher Scientific's) commercial cycle sequencing kit using four spectrally distinct dichlororhodamine dyes coupled to the four ddNTPs. The standard chemistry for capillary-based Sanger sequencing in forensic mtDNA analysis, specified by SWGDAM and ENFSI guidelines.

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Related terms

2',3'-dideoxynucleotide triphosphate (ddNTP)Cycle sequencingEMPOP (ENBISI Mitochondrial Population Database)Phred quality scorerCRS (revised Cambridge Reference Sequence)Sequencing-by-synthesis (SBS)

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