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Barcode Gap

Definition

A barcode gap is separation between observed within-taxon and between-taxon genetic variation for a specified marker and reference dataset. Its presence and size depend on sampling and the taxa examined rather than providing one universal threshold for species identification.

Context
Species identification

For example, apparent separation in a small reference set can disappear when additional close relatives or within-species variants are represented.

The best reference match is not automatically the true species. Where resolution is limited, report the supported family, genus or complex instead of forcing a species name.

Related terms

Barcode
A selected genetic region used for taxonomic comparison with appropriate references. A taxonomic result depends on marker performance, reference quality and the...
Introgression
Transfer of genetic material across population or species boundaries through hybridisation and subsequent breeding. Mitochondrial markers can therefore be shared despite other...
Metabarcoding
Sequencing a mixture of DNA from many organisms simultaneously and using bioinformatics to assign each read to a taxon. For plant eDNA,...
Voucher
A documented specimen linked to observations or reference data and available for appropriate checking. Retained, documented material can support identification and later...

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