Radioimmunoassay (RIA)
Definition
An immunological quantification method developed in the late 1950s in which a radiolabelled antigen competes with unlabelled antigen for antibody binding sites. Highly sensitive but requires radioactive isotopes and specialised facilities.
Related terms
- ABO blood group system
- Classification of red blood cells by the presence or absence of A and B antigens on the cell membrane, determined by alleles...
- Enzyme-linked immunosorbent assay (ELISA)
- An immunoassay format in which an enzyme-conjugated antibody produces a measurable colour change on substrate addition. Developed in 1971, adopted in forensic...
- Lateral-flow immunoassay
- A point-of-care competitive immunochromatographic format on a nitrocellulose strip. In the absence of drug, colloidal gold-labelled antibody is captured at a test...
- Monoclonal antibody
- An antibody produced from a single B-cell clone, recognising a single specific epitope. In forensic species identification, monoclonal antibodies offer higher specificity...
- Precipitin test
- A serological method in which an antiserum specific to one species' proteins is mixed with an extract of an unknown specimen. Formation...
Explained in
- History of Forensic Serology and Immunological MethodsAn immunological quantification method developed in the late 1950s in which a radiolabelled antigen competes with unlabelled antigen for antibody binding sites...