Enzyme-linked immunosorbent assay (ELISA)
Definition
An immunoassay format in which an enzyme-conjugated antibody produces a measurable colour change on substrate addition. Developed in 1971, adopted in forensic laboratories from the 1980s for body-fluid identification, species determination, and drug detection.
Related terms
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- Classification of red blood cells by the presence or absence of A and B antigens on the cell membrane, determined by alleles...
- Lateral-flow immunoassay
- A point-of-care competitive immunochromatographic format on a nitrocellulose strip. In the absence of drug, colloidal gold-labelled antibody is captured at a test...
- Monoclonal antibody
- An antibody produced from a single B-cell clone, recognising a single specific epitope. In forensic species identification, monoclonal antibodies offer higher specificity...
- Precipitin test
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- Radioimmunoassay (RIA)
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Explained in
- History of Forensic Serology and Immunological MethodsAn immunoassay format in which an enzyme-conjugated antibody produces a measurable colour change on substrate addition. Developed in 1971, adopted in forensic...