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Prostate-specific antigen (PSA)

Definition

A serine protease glycoprotein (also called p30) secreted in high concentrations by the prostate gland into seminal plasma. The primary immunological marker for semen in forensic casework. Detected by antibody-based assays including ELISA and lateral-flow immunochromatographic strips.

Related terms

Alpha-amylase (salivary isoform)
An enzyme present in saliva at concentrations several orders of magnitude higher than in other body fluids. Detected immunologically using anti-human salivary...
Azoospermia
The absence of spermatozoa from ejaculated semen, resulting from obstructive causes (vasectomy, ductal blockage) or non-obstructive causes (testicular failure). Azoospermic semen contains...
Differential extraction
A two-step DNA extraction procedure used when a stain contains both sperm cells and non-sperm epithelial cells. The first step lyses epithelial...
Inhibition agglutination assay
A serological technique for detecting soluble antigens in body-fluid extracts. Known antibody is incubated with the stain extract; if the target antigen...
Lateral-flow immunochromatographic strip
A point-of-care format immunoassay in which a sample migrates along a nitrocellulose membrane past a labelled antibody zone and a capture antibody...
Salivary amylase
An enzyme secreted by the parotid, submandibular, and sublingual salivary glands that cleaves starch molecules. Its high concentration in saliva relative to...
Secretor
An individual whose ABO blood group antigens are expressed on red blood cells and also released into body fluids such as saliva,...
Sexual assault forensic examination (SAFE) kit
The standardised collection kit used by trained healthcare providers to obtain biological samples from a survivor within a defined collection window, typically...
Substrate control
A sample of the unstained surface material from an area adjacent to a body fluid stain, collected alongside the stain and processed...
Touch DNA
Minute quantities of epithelial cells transferred by skin contact with a surface, without leaving a visible stain. Collected by swabbing contact points...

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