STR Profiling and Multiplex Kits (CODIS, PowerPlex, GlobalFiler): Application (UGC-NET Unit III)

Medium-band UGC-NET Forensic Science Unit III drill on short tandem repeat (STR) profiling and the multiplex amplification kits in routine forensic use. The set covers the structure of STR loci (tetranucleotide repeats of 4 bp, with dinucleotide, trinucleotide and pentanucleotide variants), the CODIS 13 original core loci (1997) and the expansion to the CODIS 20 core loci (1 January 2017: addition of D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433 and D22S1045), amelogenin for sex typing, and the routine multiplex kits in Indian and international DNA laboratories (PowerPlex Fusion 6C, GlobalFiler, Investigator 24plex, Yfiler Plus). Dye-label chemistry (6-FAM, VIC, NED, TAZ, SID with LIZ as size standard), capillary electrophoresis on the ABI 3500 and 3500xL with POP-4 polymer, allele calling against an allelic ladder, stutter peaks at n minus 4 bp, heterozygous peak height ratio above 60 percent, and locus dropout thresholds are tested in applied scenario style. Indian context covers the DNA Data Bank governance under DBT and ICMR, the DNA Profiling Division at CFSL Hyderabad, the NFSU forensic DNA laboratory, and Y-STR work in sexual assault and mass-disaster victim identification.