DNA Extraction Methods (Organic, Chelex, Magnetic Bead, FTA Paper): Application (UGC-NET Unit III)
Published:
Questions
30
Duration
30 min
Faculty-reviewed
0
Updated
17 May 2026
About this mock
UGC-NET Forensic Science Unit III drill on DNA extraction at the application band. Scenarios cover organic phenol-chloroform-isoamyl extraction with proteinase K and SDS lysis, Chelex 100 chelation chemistry, silica-coated paramagnetic bead workflows (PrepFiler, DNA IQ) on automated platforms (Qiagen EZ1, QIAcube), Whatman FTA card storage and punch processing, the Gill and Werrett differential extraction for mixed sexual-assault stains with DTT release of sperm DNA, ethanol-isopropanol precipitation, and qPCR quantitation with Quantifiler Trio and Plexor HY. Sample-type matching covers buccal swabs, bloodstains, low-template touch DNA, old skeletal remains, and inhibitor-laden specimens. Indian context covers CFSL DNA divisions running EZ1 Advanced XL automation.
Sources & references
Questions in this mock are written and verified against the following sources. Citations are recorded per question and shown in the explanation after submission.
- cited in 5 questions
Sambrook J, Russell D W, Molecular Cloning: A Laboratory Manual, 3rd edition, CSHL Press
Appendix 8, properties of SDS in cell lysis and protein denaturation
Open source - cited in 4 questions
Walsh P S, Metzger D A, Higuchi R, Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material, BioTechniques 10(4):506-513 (1991)
Butler, Fundamentals of Forensic DNA Typing, Chapter 4 on Chelex single-stranded output
Open source - cited in 2 questions
Butler J M, Fundamentals of Forensic DNA Typing, Academic Press
Chapter 4, Sample collection, storage and DNA extraction; differential extraction protocol of Gill and Werrett
Open source - cited in 2 questions
Qiagen, EZ1 DNA Investigator Handbook and EZ1 Advanced XL User Manual
Validation studies cited by Shrivastava et al, CFSL Hyderabad and CDFD DNA Fingerprinting Unit publications on automated extraction
Open source - cited in 2 questions
Cytiva (formerly GE Healthcare Whatman), FTA Card Care, Storage and Sample Preparation Technical Bulletin
Punch yields and direct-amplification kit input requirements documented in Thermo Fisher GlobalFiler Express User Guide
Open source - cited in 1 question
Ebeling W et al, Proteinase K from Tritirachium album Limber, European Journal of Biochemistry 47(1):91-97 (1974)
Enzyme properties and use in nucleic-acid isolation reviewed in Sambrook and Russell, Molecular Cloning 3rd edition
Open source - cited in 1 question
SWGDAM Interpretation Guidelines for Autosomal STR Typing by Forensic DNA Testing Laboratories (2017, revised 2024)
ENFSI DNA Working Group, Recommended Minimum Criteria for the Validation of Various Aspects of the DNA Profiling Process
Open source - cited in 1 question
Promega Corporation, Plexor HY System Technical Manual TM293
Iso-dC/iso-dGTP chemistry described in Sherrill et al, Journal of the American Chemical Society 126 (2004) 4550-4556
Open source - cited in 1 question
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Volume 73, Chloroform
Safety profile cross-referenced with Butler, Fundamentals of Forensic DNA Typing, Chapter 4 on organic extraction
Open source - cited in 1 question
Burgoyne L A, US Patent 5,496,562, Solid medium and method for DNA storage (1996, Whatman FTA)
Cited in Butler, Fundamentals of Forensic DNA Typing, Chapter 4, and in Whatman FTA Card user technical bulletins
Open source - cited in 1 question
Burgoyne L A, US Patent 5,496,562 and Cytiva (Whatman) FTA Card technical bulletins on long-term storage stability
Criminal Procedure (Identification) Act 2022 read with the FBI CODIS and Indian State DNA Index System SOPs on reference-sample formats
Open source - cited in 1 question
Bureau of Police Research and Development (BPR&D), Modernisation Plan for State Forensic Science Laboratories, DNA Division annexure
ENFSI DNA Working Group, Modernisation and Automation in Forensic DNA Workflows, position paper
Open source - cited in 1 question
Thermo Fisher Scientific, PrepFiler Forensic DNA Extraction Kit User Guide, Publication 4463273
PrepFiler BTA chemistry documented in the AutoMate Express User Guide
Open source - cited in 1 question
Akane A et al, Identification of the heme compound copurified with DNA from bloodstains, Journal of Forensic Sciences 39(2):362-372 (1994)
Inhibitor-tolerance comparisons in Alaeddini R, Forensic Science International: Genetics 6 (2012) 297-305
Open source - cited in 1 question
Gill P, Jeffreys A J, Werrett D J, Forensic application of DNA fingerprints, Nature 318 (1985) 577-579
Differential extraction lysis composition reviewed in Butler, Fundamentals of Forensic DNA Typing, Chapter 4
Open source - cited in 1 question
Boom R et al, Rapid and simple method for purification of nucleic acids, Journal of Clinical Microbiology 28(3):495-503 (1990)
Foundational silica-chaotrope chemistry underlying PrepFiler and DNA IQ
Open source - cited in 1 question
Promega Corporation, DNA IQ System Database Protocol Technical Manual TM296
Forensic-casework protocols and Maxwell 16 implementation referenced in Butler, Fundamentals of Forensic DNA Typing, Chapter 4
Open source - cited in 1 question
Thermo Fisher Scientific, Quantifiler HP and Quantifiler Trio DNA Quantification Kits User Guide, Publication 4485354
SA, LA, T-large and IPC assay design described in Holt et al, Forensic Science International: Genetics 21 (2016) 145-157
Open source - cited in 1 question
Alaeddini R, Forensic implications of PCR inhibition: A review, Forensic Science International: Genetics 6 (2012) 297-305
Quantifiler Trio IPC interpretation discussed in Thermo Fisher Publication 4485354
Open source - cited in 1 question
Loreille O M et al, High efficiency DNA extraction from bone by total demineralisation, Forensic Science International: Genetics 1(2):191-195 (2007)
PrepFiler BTA Forensic DNA Extraction Kit User Guide (Thermo Fisher), validated for bone, teeth and adhesive samples
Open source
How our mocks are built
Questions are written and edited by the ForensicSpot team and cited from peer-reviewed forensic textbooks, official syllabi and primary case law. Each one is verified before publishing. Detailed explanations show after you submit, so the test stays a real test. See a mistake? Tell us.
Common questions
What does the DNA Extraction Methods (Organic, Chelex, Magnetic Bead, FTA Paper): Application (UGC-NET Unit III) mock cover?+
UGC-NET Forensic Science Unit III drill on DNA extraction at the application band. Scenarios cover organic phenol-chloroform-isoamyl extraction with proteinase K and SDS lysis, Chelex 100 chelation chemistry, silica-coated paramagnetic bead workflows (PrepFiler, DNA IQ) on automated platforms (Qiagen EZ1, QIAcube), Whatman FTA card storage and punch processing, the Gill and Werrett differential extraction for mixed sexual-assault stains with DTT release of sperm DNA, ethanol-isopropanol precipit
How many questions and how long is the test?+
30 multiple-choice questions, 30 minutes total. Difficulty: medium. Tier: Premium.
Who is this mock for?+
Forensic science students and aspirants who want timed, exam-style practice with explanations and verified source citations on NET. Useful for postgraduate entrance preparation and for BSc / MSc forensic students testing their recall under time.
Are the questions reviewed?+
Each question carries a verified source citation. Faculty review for individual questions is in progress.
Do I need an account to take this mock?+
Yes, a free ForensicSpot account is required to start a timed attempt — this lets you save progress, see per-question explanations after submission, and track your topic-level performance over time.