Chromatography: Method Selection (GLC, HPLC, HPTLC) (UGC-NET Unit II)
Published:
Questions
30
Duration
30 min
Faculty-reviewed
0
Updated
17 May 2026
About this mock
UGC-NET Forensic Science Unit II drill on chromatography at the method-selection band. Each scenario asks the candidate to choose the appropriate technique, column chemistry, mobile phase, detector, or sample preparation for a forensic analytical problem, covering cannabinoid TLC screening, opioid HPLC-UV, amphetamine GC-FID with PFPA derivatization, benzodiazepine LC-MS/MS, fire debris GC-FID under ASTM E1618, ink HPTLC, toxicology LC-HRMS, and headspace GC-FID for volatiles, alcohols, and cyanide. Companion to the foundational chromatography mock on the same syllabus area.
Sources & references
Questions in this mock are written and verified against the following sources. Citations are recorded per question and shown in the explanation after submission.
- cited in 3 questions
ASTM International, Standard Test Method E1618
Ignitable Liquid Residues in Extracts from Fire Debris Samples by Gas Chromatography-Mass Spectrometry
Open source - cited in 2 questions
SAMHSA, Mandatory Guidelines for Federal Workplace Drug Testing Programs
Marijuana Metabolite Confirmation by GC-MS, 15 ng/mL Cut-Off
Open source - cited in 1 question
Stauffer E, Dolan JA, Newman R, Fire Debris Analysis
Chapter on Analysis of Biological Samples for Accelerants
Open source - cited in 1 question
UNODC, Recommended Methods for the Identification and Analysis of Cannabis and Cannabis Products
ST/NAR/40, Manual for Use by National Drug Testing Laboratories
Open source - cited in 1 question
UNODC, Recommended Methods for the Identification and Analysis of Amphetamine, Methamphetamine and their Ring-Substituted Analogues in Seized Materials
ST/NAR/34, Manual for Use by National Drug Testing Laboratories
Open source - cited in 1 question
TIAFT, The International Association of Forensic Toxicologists, Laboratory Guidelines
Recommendations on Sample Preparation of Biological Specimens for Systematic Toxicological Analysis
Open source - cited in 1 question
Knapp DR, Handbook of Analytical Derivatization Reactions
Chapter on Trimethylsilyl Derivatisation for Gas Chromatography
Open source - cited in 1 question
Maurer HH, Position of Chromatographic Techniques in Screening for Detection of Drugs or Poisons in Clinical and Forensic Toxicology and Doping
Clinical Chemistry and Laboratory Medicine 2004; 42(11): 1310 to 1324
Open source - cited in 1 question
Logue BA et al, The Analysis of Cyanide and its Breakdown Products in Biological Samples
Critical Reviews in Analytical Chemistry 2010; 40(2): 122 to 147
Open source - cited in 1 question
Peters FT, Drummer OH, Musshoff F, Validation of New Methods
Forensic Science International 2007; 165(2-3): 216 to 224
Open source - cited in 1 question
Maurer HH, Position of Liquid Chromatography-Mass Spectrometry in Clinical and Forensic Toxicology
Analytical and Bioanalytical Chemistry 2007; 388(7): 1315 to 1325
Open source - cited in 1 question
Stahl E, Thin-Layer Chromatography, A Laboratory Handbook
Section on TLC of Alkaloids and Basic Drugs
Open source - cited in 1 question
SWGDRUG, Scientific Working Group for the Analysis of Seized Drugs, Recommendations
Part IVB Methods of Analysis / Drug Identification
Open source - cited in 1 question
Vehovec T and Obreza A, Review of Operating Principle and Applications of the Charged Aerosol Detector
Journal of Chromatography A 2010; 1217(10): 1549 to 1556
Open source - cited in 1 question
Sherma J, Review of HPTLC in Drug Analysis
Journal of Liquid Chromatography and Related Technologies 2010; 33(9-12): 1015 to 1043
Open source - cited in 1 question
UNODC, Methods for Impurity Profiling of Heroin and Cocaine
ST/NAR/35, Manual for Use by National Drug Analysis Laboratories
Open source - cited in 1 question
SWGDOC, Scientific Working Group for Forensic Document Examination, Standard for the Examination of Documents for Indented Writing and Ink Comparison
Standard for the Examination of Documents
Open source - cited in 1 question
Jones AW, Measuring and Reporting the Concentration of Ethanol in Blood and Breath for Forensic Purposes
WIREs Forensic Science 2019; 1(6): e1351
Open source - cited in 1 question
Maurer HH, Multi-analyte procedures for screening for and quantification of drugs in blood, plasma, or serum by liquid chromatography-single stage or tandem mass spectrometry (LC-MS or LC-MS/MS) relevant to clinical and forensic toxicology
Clinical Biochemistry 2005; 38(4): 310 to 318
Open source - cited in 1 question
Wille SMR et al, Practice-Oriented Workflow for Forensic Toxicology Confirmation
Journal of Analytical Toxicology 2018; 42(6): 366 to 374
Open source - cited in 1 question
WADA, World Anti-Doping Agency, Technical Document for Initial Testing and Confirmation Procedures
TD2022EAAS, Endogenous Anabolic Androgenic Steroids
Open source - cited in 1 question
Huestis MA et al, Cannabinoid Concentrations in Hair from Documented Cannabis Users
Forensic Science International 2007; 169(2-3): 129 to 136
Open source - cited in 1 question
Society of Hair Testing, Consensus on Hair Analysis
Recommendations for Hair Testing in Forensic Cases
Open source - cited in 1 question
Tagliaro F et al, Chromatographic methods for blood alcohol determination
Journal of Chromatography B 1992; 580(1-2): 161 to 190
Open source - cited in 1 question
Snyder LR, Kirkland JJ, Dolan JW, Introduction to Modern Liquid Chromatography, 3rd ed
Chapter on Gradient Elution
Open source - cited in 1 question
WADA, Technical Document TD2022EAAS
Endogenous Anabolic Androgenic Steroid Measurement and Reporting
Open source - cited in 1 question
Peters FT, Recent Advances of Liquid Chromatography Tandem Mass Spectrometry in Clinical and Forensic Toxicology
Clinical Biochemistry 2011; 44(1): 54 to 65
Open source
How our mocks are built
Questions are written and edited by the ForensicSpot team and cited from peer-reviewed forensic textbooks, official syllabi and primary case law. Each one is verified before publishing. Detailed explanations show after you submit, so the test stays a real test. See a mistake? Tell us.
Common questions
What does the Chromatography: Method Selection (GLC, HPLC, HPTLC) (UGC-NET Unit II) mock cover?+
UGC-NET Forensic Science Unit II drill on chromatography at the method-selection band. Each scenario asks the candidate to choose the appropriate technique, column chemistry, mobile phase, detector, or sample preparation for a forensic analytical problem, covering cannabinoid TLC screening, opioid HPLC-UV, amphetamine GC-FID with PFPA derivatization, benzodiazepine LC-MS/MS, fire debris GC-FID under ASTM E1618, ink HPTLC, toxicology LC-HRMS, and headspace GC-FID for volatiles, alcohols, and cyan
How many questions and how long is the test?+
30 multiple-choice questions, 30 minutes total. Difficulty: medium. Tier: Premium.
Who is this mock for?+
Forensic science students and aspirants who want timed, exam-style practice with explanations and verified source citations on NET. Useful for postgraduate entrance preparation and for BSc / MSc forensic students testing their recall under time.
Are the questions reviewed?+
Each question carries a verified source citation. Faculty review for individual questions is in progress.
Do I need an account to take this mock?+
Yes, a free ForensicSpot account is required to start a timed attempt — this lets you save progress, see per-question explanations after submission, and track your topic-level performance over time.