Body Fluids Identification (Seminal, Saliva, Urine, Sweat, Vaginal): Foundations (UGC-NET Unit III)
Published:
Questions
30
Duration
30 min
Faculty-reviewed
0
Updated
17 May 2026
About this mock
UGC-NET Forensic Science Unit III drill on identification of seminal fluid, saliva, urine, sweat, vaginal secretions, menstrual blood and faecal traces at the foundations level. Covers presumptive screens (Florence iodine for choline, Barberio for spermine, acid phosphatase with Brentamine Fast Blue, alpha-amylase Phadebas, thiocyanate, Lugol's iodine), confirmatory tests (Christmas-tree stain for sperm heads, RSID-Semen and ABAcard p30 for PSA, SVSA for semenogelin, RSID-Saliva, D-dimer ELISA for menstrual blood, Edelman test for urobilinogen), and the Indian CFSL serology workflow. Easy-band questions calibrated for first-pass UGC-NET preparation and quick concept refresh.
Sources & references
Questions in this mock are written and verified against the following sources. Citations are recorded per question and shown in the explanation after submission.
- cited in 12 questions
Gaensslen R E, Sourcebook in Forensic Serology, Immunology, and Biochemistry
Saferstein R, Criminalistics: An Introduction to Forensic Science, Chapter on forensic serology: presumptive and confirmatory tests for seminal stains
- cited in 3 questions
Magnusson Diagnostics, Phadebas Forensic Press Test User Manual
Quarino L, Dang Q and Hartmann J, An ELISA method for the identification of salivary amylase, Journal of Forensic Sciences 2005
- cited in 3 questions
ENFSI Body Fluids Working Group, Best Practice Manual for the Identification of Body Fluids
Saferstein R, Forensic Science Handbook, Volume II, Chapter on serological identification: presumptive vs confirmatory testing
- cited in 2 questions
Allery J P et al, Cytological detection of spermatozoa: comparison of three staining methods, Journal of Forensic Sciences 2001
FBI Laboratory, Biological Evidence Field Guide, Section on Christmas-tree staining for spermatozoa
- cited in 2 questions
Independent Forensics, RSID-Semen Lateral Flow Immunochromatographic Test User Guide
Pang B C M and Cheung B K K, Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen, Forensic Science International 2007
- cited in 2 questions
Saferstein R, Criminalistics: An Introduction to Forensic Science
Pearson, 12th Edition, Chapter on acid phosphatase screening for seminal stains
- cited in 1 question
Akutsu T et al, Detection of D-dimer for the identification of menstrual blood, Legal Medicine 2018
Gaensslen R E, Sourcebook in Forensic Serology, Immunology, and Biochemistry
- cited in 1 question
Indian Council of Medical Research, Standards on Sexual Assault Examination and Evidence Collection (SAFE kit guidelines)
Directorate of Forensic Science Services, MHA, Manual on Forensic Serology Procedures at CFSL
- cited in 1 question
Pang B C M and Cheung B K K, Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen, Forensic Science International 2007
Independent Forensics, RSID-Semen Lateral Flow Immunochromatographic Test User Guide
- cited in 1 question
Boskey E R et al, Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source, Human Reproduction 2001
Hanssen E N et al, Body fluid prediction from microbial patterns for forensic application, Forensic Science International Genetics 2018
- cited in 1 question
Abacus Diagnostics, ABAcard p30 Test for the Forensic Identification of Semen, Product Insert
Hochmeister M N et al, Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid, Journal of Forensic Sciences 1999
- cited in 1 question
Independent Forensics, RSID-Saliva Lateral Flow Immunochromatographic Test User Guide
Old J et al, Developmental validation of RSID-Saliva: a lateral flow immunochromatographic strip test for the forensic detection of saliva, Journal of Forensic Sciences 2009
How our mocks are built
Questions are written and edited by the ForensicSpot team and cited from peer-reviewed forensic textbooks, official syllabi and primary case law. Each one is verified before publishing. Detailed explanations show after you submit, so the test stays a real test. See a mistake? Tell us.
Common questions
What does the Body Fluids Identification (Seminal, Saliva, Urine, Sweat, Vaginal): Foundations (UGC-NET Unit III) mock cover?+
UGC-NET Forensic Science Unit III drill on identification of seminal fluid, saliva, urine, sweat, vaginal secretions, menstrual blood and faecal traces at the foundations level. Covers presumptive screens (Florence iodine for choline, Barberio for spermine, acid phosphatase with Brentamine Fast Blue, alpha-amylase Phadebas, thiocyanate, Lugol's iodine), confirmatory tests (Christmas-tree stain for sperm heads, RSID-Semen and ABAcard p30 for PSA, SVSA for semenogelin, RSID-Saliva, D-dimer ELISA f
How many questions and how long is the test?+
30 multiple-choice questions, 30 minutes total. Difficulty: easy. Tier: Premium.
Who is this mock for?+
Forensic science students and aspirants who want timed, exam-style practice with explanations and verified source citations on NET. Useful for postgraduate entrance preparation and for BSc / MSc forensic students testing their recall under time.
Are the questions reviewed?+
Each question carries a verified source citation. Faculty review for individual questions is in progress.
Do I need an account to take this mock?+
Yes, a free ForensicSpot account is required to start a timed attempt — this lets you save progress, see per-question explanations after submission, and track your topic-level performance over time.