Sandwich immunoassay
Definition
The detection architecture used by most lateral-flow strips for large antigens: the target antigen is captured between two antibodies, the conjugated detection antibody and the immobilised capture antibody at the test line. Both antibodies must bind simultaneously for a signal to appear.
Related terms
- ABAcard HemaTrace
- A commercial lateral-flow strip that detects human haemoglobin alpha-chain using a monoclonal antibody. Validated for forensic blood identification, with documented cross-reactivity to...
- Colloidal gold
- Nanometre-scale gold particles that appear red or pink in visible light. Widely used as the reporter particle in lateral-flow strips because they...
- Conjugate pad
- A zone upstream of the test and control lines containing dried detection antibodies pre-bound to colloidal gold or coloured latex particles. On...
- Nitrocellulose membrane
- The porous polymer strip along which sample fluid wicks by capillary action. The test and control line antibodies are immobilised directly on...
- RSID (Rapid Stain Identification)
- A series of body-fluid-specific lateral-flow strips produced by Independent Forensics, with separate products targeting semenogelin (semen), salivary alpha-amylase (saliva), MMP-10 and related...
Explained in
- Lateral-Flow and Immunochromatographic Assays in Forensic TestingThe detection architecture used by most lateral-flow strips for large antigens: the target antigen is captured between two antibodies, the conjugated detection...