RT-qPCR
Definition
Reverse-transcription quantitative PCR: a two-step assay in which RNA is first converted to complementary DNA (cDNA) by reverse transcriptase, then amplified and quantified by real-time PCR. The cycle threshold (Ct) value is inversely proportional to the initial RNA abundance.
Related terms
- Degradation index
- A ratio or combination of RNA abundance measurements designed to capture the state of degradation of a sample. Often calculated as the...
- Inter-individual variability
- Differences in RNA degradation rate between different donors' blood, arising from variation in RNase activity, blood cell composition, and haematological status. One...
- microRNA (miRNA)
- Small non-coding RNA molecules of approximately 18-24 nucleotides that regulate gene expression post-transcriptionally. Abundant in blood cells, relatively resistant to degradation compared...
- mRNA (messenger RNA)
- Transcripts encoding proteins, synthesised in the nucleus from DNA templates and translated by ribosomes. mRNA molecules vary greatly in length (hundreds to...
- RNase
- Ribonuclease enzymes that cleave RNA phosphodiester bonds. Present in blood plasma (RNase A family) and released from cells on death. The activity...
Explained in
- mRNA and microRNA Approaches to Stain AgeReverse-transcription quantitative PCR: a two-step assay in which RNA is first converted to complementary DNA (cDNA) by reverse transcriptase, then amplified a...