Skip to content

Prostate-Specific Antigen (PSA / P30)

Definition

A glycoprotein produced by the prostate gland and secreted into seminal plasma at high concentrations. The PSA immunochromatographic strip test is the standard confirmatory test for semen in many national laboratories and is valid even when no spermatozoa are recovered.

Related terms

ABAcard P30 (HemaTrace P30)
A lateral-flow immunochromatographic card that detects PSA at approximately 0.2-4 ng/mL of extract. Widely used in sexual assault kit triage because it...
Acid Phosphatase (AP)
An enzyme present at high concentrations in seminal plasma. The Brentamine Fast Violet B (or SERI AP) spray test detects acid phosphatase...
Amylase
An enzyme that breaks down starch and is present at high activity in saliva. The Phadebas press test and starch-iodine method detect...
Confirmatory Test
A test with sufficient specificity to establish the identity of a biological fluid for reporting purposes. Examples include Takayama and Teichmann crystal...
Luminol
A chemiluminescent reagent (5-amino-2,3-dihydro-1,4-phthalazinedione) that produces blue-white light on contact with haemoglobin-derived iron. Detects blood diluted to 1:10,000,000 or more. Used as...
Presumptive Test
A fast, low-cost, class-specific screen used to triage a caseload before instrumental work begins. Examples are Marquis reagent for opioids and amphetamines,...
RSID-Semen
Rapid Stain Identification of Semen: an immunochromatographic lateral-flow strip from Independent Forensics that detects semenogelin I and II with sensitivity to approximately...
Semenogelin I and II
High-molecular-weight proteins secreted by the seminal vesicles that form the semen coagulum immediately after ejaculation. PSA then cleaves them to liquefy the...
Sensitivity and Specificity in Forensic Context
Sensitivity is the probability of a positive result when semen is truly present; specificity is the probability of a negative result when...
Seratec PSA Semiquant
A lateral-flow PSA strip with a semi-quantitative band-intensity scale. Line intensity corresponds approximately to 1 ng/mL, 2 ng/mL, or greater concentrations, useful...

Explained in these topics

  • Biological Sample Screening and Presumptive TestsA glycoprotein produced by the prostate gland and secreted into seminal plasma at high concentrations. The PSA immunochromatographic strip test is the standard...
  • p30/PSA and RSID-SemenA 33-kDa serine protease encoded by the KLK3 gene, secreted by prostate epithelial cells into seminal plasma at 0.5-2 mg/mL. Detected in forensic stain extract...

Your journey to becoming a forensic professional starts here.

Practice with mock tests, learn from structured notes, and get your questions answered by a global forensic community, all in one place.