Low copy number (LCN) DNA
Definition
An approach to analysing low-template samples by increasing the number of PCR amplification cycles, typically to 34 cycles. Increases sensitivity but also amplifies contaminants and stochastic noise, requiring strict quality controls and replicate analysis.
Related terms
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- A terminally differentiated, anucleate cell forming the outermost skin layer (stratum corneum). Corneocytes shed continuously. Nucleated cells from deeper epidermal layers are...
- Double-swab technique
- A collection method for dried biological stains in which a moistened swab rehydrates and lifts the stain from the surface, followed immediately...
- Secondary transfer
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- Stochastic effect
- A random sampling artefact that occurs at very low template quantities. Examples include allelic dropout (an allele fails to amplify), allelic drop-in...
- Touch DNA
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Explained in
- Touch DNA and Trace Biological MaterialAn approach to analysing low-template samples by increasing the number of PCR amplification cycles, typically to 34 cycles. Increases sensitivity but also ampl...