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Environmental SEM (ESEM)

Definition

Variant of conventional SEM that runs the sample chamber at higher pressure (up to several torr of water vapour) instead of the standard 10^-5 torr vacuum. Allows imaging of hydrated, oily or volatile samples without dehydration, including some biological tissues and uncoated polymer surfaces. The trade-off is reduced resolution and reduced EDS performance because of beam scattering in the gas.

Related terms

ASTM E1588
ASTM International standard practice for gunshot residue analysis by scanning electron microscopy with energy-dispersive spectrometry. Defines particle classification (characteristic, consistent-with, indicative), morphology...
Back-Scattered Electron (BSE)
High-energy electron from the primary beam that has bounced elastically off a nucleus and exited the sample. BSE yield increases with atomic...
EDS / EDXRF Detector
Silicon-drift detector mounted at a fixed take-off angle to the sample that reads characteristic X-rays emitted as the primary beam ionises atoms...
Secondary Electron (SE)
Low-energy electron (below 50 eV) ejected from the top few nanometres of the sample when the primary beam knocks a loosely bound...
Sputter Coating
Vacuum-deposition technique that lays a thin (5 to 15 nm) conductive layer of gold, gold-palladium or carbon onto a non-conductive sample (polymer,...

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