Skip to content

Anion Gap

Definition

Sodium minus the sum of chloride and bicarbonate in serum. A widened anion gap (above 16) in a comatose patient with unexplained acidosis is the bedside clue to methanol or ethylene glycol.

Formula
Na - (Cl + HCO3)
Alert threshold
Above 16 is widened
Clinical use
Bedside screen for toxic alcohol ingestion
Sample
Serum electrolytes

Common questions

Why does a widened anion gap point toward methanol or ethylene glycol rather than another cause?+

Many conditions widen the anion gap, but in a comatose patient with unexplained acidosis and no obvious cause such as diabetic ketoacidosis or renal failure, toxic alcohol metabolites accumulating as unmeasured acids become a leading suspect that prompts specific confirmatory testing.

Can the anion gap alone confirm methanol poisoning?+

No, it is a rapid screening clue, not a confirmatory test. Definitive identification requires measuring methanol and its toxic metabolite formate directly, typically by gas chromatography, since other conditions can also widen the gap.

Related terms

Carboxyhaemoglobin (COHb)
The compound formed when carbon monoxide binds to haemoglobin at the oxygen-binding site, with an affinity 200-250 times greater than oxygen. Produces...
Cherry-Pink Lividity
The bright pink discolouration of dependent tissues seen in CO and cyanide deaths. In CO the colour comes from COHb itself, in...
Conway Microdiffusion
A two-chamber glass cell in which an acid in the outer ring liberates HCN from the sample and the gas diffuses into...
Headspace GC
Gas chromatography in which the sample (blood, urine, vitreous) is sealed in a vial, equilibrated at a fixed temperature, and a portion...
Saturation Kinetics
Enzyme behaviour at substrate concentrations above the Km, where the rate of metabolism is constant rather than proportional to concentration. Ethanol saturates...

Explained in

Your journey to becoming a forensic professional starts here.

Practice with mock tests, learn from structured notes, and get your questions answered by a global forensic community, all in one place.